Cloning Nanobody cDNA into pHEN6c Plasmid Vector

Plasmid vector for cloning infectious cDNAs from plant RNA viruses: high infectivity of cDNA clones of tomato aspermy cucumovirus.

An improved version of the previously obtained cloning vector pCass was constructed by partially duplicating the 35S promoter used to drive the transient transcription of cloned viral cDNAs. Full-length cDNAs of the three genomic RNAs of tomato aspermy cucumovirus (TAV) cloned in this improved pCass (designated pCass2) gave a 3-fold higher infectivity in two plant species tested than the same c...

Cloning of Rotavirus Outer Capsid Protein (VP7) Gene into the pGEM Vector

Cloning of Rota Virus Outer Capsid Protein (VP7) Gene into the pGEM Vector

Background and Aims: In humans the group A rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. Introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...

Reliable PCR-based method for cloning cDNA in plasmid vectors by frequency estimation.

products by generating sticky ends for ligation. This step is not essential and should be omitted when using a bluntcutting restriction endonuclease that is inhibited by methylation. The results from applying our approach demonstrate the mutation of three bases of a HBV sequence using an alternative PCR-based, SDM method. The technique obviates the variable efficiency of using doublestranded me...

Lecture 11 - cDNA Cloning Lab

Research Objective A researcher used differential cDNA cloning to isolate a cDNA transcripts representing growth factor induced genes. One of these cDNA clones corresponded to a previously uncharacterized open reading frame in the human genome which the exasperated researcher named "Another Moronic Gamble" (AMG). Since increased steady-state mRNA levels could be due to either transcriptional in...